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. 2021 Aug 8;297(3):101049. doi: 10.1016/j.jbc.2021.101049

Figure 1.

Figure 1

FUS promotes cell proliferation.A, schematic of the FUS gene targeting. Two guide RNAs, sgRNA1 and sgRNA2, were used to target FUS exon 4 (see Experimental procedures section). B, expression of FET proteins (FUS, EWSR1, and TAF15) in FUS−/− clones. C, reconstitution of FUS−/− (Cl.110) with an untagged FUS retroviral vector. The same vector expressing β-glucuronidase (GUS) was introduced as a negative control into FUS−/− cells. D, FUS−/− cell colonies exhibited reduced growth relative to FUS+/+ and FUS−/−:FUS cells. E, cell proliferation rates of FUS+/+, FUS−/−, and FUS−/−: FUS U-2 OS cells. Three biological replicates were used. The bars represent mean ± SE. The two-way ANOVA test was performed, and the p values shown on plot are adjusted p values by Tukey's multiple comparisons test. FUS, fused in sarcoma.