Figure 7.

FUS regulates DNA replication timing (RT).A, asynchronous U-2 OS cells and three FUS^−/− clones (Cl. 46, Cl. 65, and Cl. 110) were pulse labeled with EdU for 20 min and scored for the presence of early, mid, or late EdU staining patterns. B, quantification analysis of cell numbers of each S-phase patterns in (A) and the percentages were calculated in each sample. C, cells were synchronized with double thymidine and then released into S phase for indicated times. Cells were then pulse labeled with BrdU, stained, and imaged by confocal microscopy. D, quantification results of samples using a minimum 100 cells per sample (C). BrdU, 5-bromo-2′-deoxyuridine; EdU, 5-ethynyl-2′-deoxyuridine; FUS, fused in sarcoma.