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Bright-field imaging of ISHs and fluorescent imaging of FISHs and IHCs were performed using the following microscopes:
| Microscope | Objectives | Software | Purpose |
|---|---|---|---|
| Zeiss Axio Scan.Z1 slidescanner | 5×/0.25 Fluar air | ZEN 2 slidescan (blue edition) | Bright field images of chromogenic ISHs |
| 10×/0.45 Plan-Apochromat air | |||
| Zeiss LSM 800 with Airyscan/confocal | 25×/0.8 Plan-Neofluar oil | ZEN 2.6 (blue edition) | Overview and analysis images of multiplex FISH |
| 40×/1.4 Plan-Apochromat oil |
