Figure 4.

Upregulation of WNT3A alleviates repressed osteogenic differentiation of hPDLF promoted by miR-874-3p. (A) The expression levels of WNT3A, β-catenin and ABC protein in cells transfected with either miR-NC or miR-874-3p mimics with either WNT3A overexpressing vector or vector control were determined by Western blot. β-actin was applied as the endogenous reference genes. (B) ALP activity and (C) Alizarin red staining was applied to detect the degree of osteogenic differentiation of hPDLF for each group. ∗∗p < 0.01 versus control group and ^##p < 0.01 versus LPS alone group. Data are presented as mean ± SD.