Fig. 2.

Inhibition of cell division in single cells expressing different levels of GFP-SAMD9L fusion protein. Dilution of CTV was used to measure cell division by 293FT cells labeled with CTV 3 h prior to transfection or treatment with the microtubule inhibitor nocodazole as a positive control. Cells were either mock-transfected or transfected with vectors encoding N-terminal GFP-tagged SAMD9L, either with WT sequence, the missense variant Arg986Cys, or the Asn885fs and Lys878fs truncating variants from P1 or P2, and analyzed 72 h later by flow cytometry. (A) Representative flow cytometric plots showing CTV and GFP fluorescence in individual cells. (B) GFP geometric mean fluorescence intensity (MFI, error bars 95% CI) gated on all GFP-positive cells. (C) CTV geometric MFI in cells gated as shown in A, either GFP-negative cells or cells with comparable, intermediate levels of GFP-SAMD9L fusion protein (GFP Int). Results are representative of three independent experiments.