Schematic relationship of sunset and moonrise time regulate the spawning behavior in D. speciosa. (A) Sunset and moonrise time in Lyudao during the spawning season, periods from April 4th to April 12th (4 d before full moon to 4 d after full moon), 2020, were shown as an example. Moonrise occurs prior to sunset before the full moon day (April 8th), whereas moonrise occurs following sunset after the full moon day. Note that sunset time changes from 18:11 on April 4th to 18:14 on April 12th, and moonrise time changes from 14:11 on April 4th to 22:48 on April 12th. (B) Before the full moon day, the presence of moonlight after sunset suppresses the process of spawning. (C) After the full moon day, the presence of darkness after sunset triggers the process of spawning.