Fig. 1.

14-3-3ζ KO rats exhibit severe and early IA. (A) The WT (n = 8) and KO animals (n = 8) were subjected to PIA. The experiment was repeated at least three times. Representative body weight gain and IA score during PIA are shown. (B) Representative pictures showing inflamed joint in WT versus KO animals are shown. (C) The 3D reconstruction of μCT scans of ankle and knee joints are shown. (Scale bar: 1 mm.) (D) Hematoxylin and eosin (H&E) staining of ankles harvested from WT or 14-3-3ζ KO rats were compared for bone damage and infiltrating immune cells. (Scale bar: 200 µm.) (E) The 14-3-3ζ antibody levels in synovial fluid were measured in WT and KO animals using ELISA. (F and G) Plasma profile of IL-17A and IL-6 measured in WT and KO animals using ELISA.