Fig. 4.

Wnt signaling promotes chromosome congression and alignment through KIF2A. (A) Representative time lapses of mitotic HeLa cells stably expressing EB3-EGFP, which marks the microtubule plus ends and centrosomes. Cells were transfected with the indicated siRNAs and stained with SiR-DNA before imaging to label the chromosomes. (B) Analysis of congression and alignment delay in n = 20 HeLa cells from A. (C) Analysis of mitotic progression from NEB to anaphase onset in HeLa cells from A. (D–I) Quantification of chromosome alignment in mitotic HeLa cells transfected with siRNA, cotransfected with empty or EGFP-KIF2A plasmid or treated as indicated for 1.5 h. In D, F, and H, representative immunofluorescence microscopy images of n ≥ 2 independent experiments are shown. In E, G, and I, the length occupied by the chromosomes at the metaphase plate was divided by the width toward the spindle poles in n > 35 mitotic cells per condition. All single cells and the median values are shown.