Fig. 2.
Effects of extracellular A9C on whole-cell TMEM16A, TMEM16A-Q645A, and -I637A currents in the absence or presence of intracellular Ca2+. (A) Whole-cell currents recorded from HEK-293T cells expressing TMEM16A, TMEM16A-Q645A, or -I637A channels in the absence (control) or presence of 300 µM [A9C]ext and the indicated [Ca2+]i. The stimulation protocol is shown in the Upper Left. Dashed horizontal lines represent the zero-current level. (B) Mean-peak (i) or steady-state (ii) whole-cell current density versus Vm relationships measured in the absence or presence of 300 µM [A9C]ext and various [Ca2+]i, for the various channel types, as indicated. The number of experiments was 6 to 10 in each case.