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. 2021 Aug 10;134(15):jcs240135. doi: 10.1242/jcs.240135

Fig. 3.

Fig. 3.

CD99 suppresses breast cancer migration in vitro. (A) Real-time migration of CD99 siRNA-treated MDA-MB-231 cells determined using the modified Boyden chamber CIM plates and xCelligence RTCA. Changes in impedance are indicative of the number of cells that have migrated. Impedance was measured every 15 min for 24 h. n=4 independent experiments. (B) Representative images of scratch wound migration assay. MDA-MB-231-GFP cells were treated with the indicated individual CD99 siRNA duplexes, pooled CD99 siRNA or control siRNA for 72 h and seeded at equal density into 96-well plates until confluent. Cells were serum starved for 2-3 h before ‘wounding’ using a Wound Maker tool (Essen Bioscience). Migration of siRNA-treated MDA-MB-231 cells was imaged at 0 and 20 h and quantified using ImageJ. (C) Quantification of B, from n=5 independent replicates. Data are mean±s.e.m. *P<0.05, **P<0.005, ***P<0.0005, ****P<0.0001 [analysis of covariance (A) and one-way ANOVA with Tukey's multiple comparison (C)].