Figure 4.

Oncogenic APC truncation and impaired AXIN1 polymerization limit DC activity

(A and C) In vitro DC activity assays in the presence of 5 μM GSK3 inhibitor. Data are displayed as in Figure 3D. APC and AXIN1 mutant variants were used as indicated.

(B and D) Quantification of the APC-dependent poly-ubiquitylation of β-catenin. Data are means from three independent experiments ± SEM.

Statistical analyses were performed using one-way ANOVA with the Bonferroni test for multiple comparisons. Asterisks above bars (without brackets) refer to comparisons to the no-APC condition (black bar). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; ns, not significant. See Tables S6 and S8 for details of statistical analyses, Figure S5 for data from the 60 min time point and protein quality control, and Figures S6B and S6C for analysis of poly-ubiquitin linkage type.