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. 2021 Aug 19;81(16):3246–3261.e11. doi: 10.1016/j.molcel.2021.07.013

Figure 5.

Figure 5

AXIN1 polymerization and APC regulate kinase activity in the DC

Kinetic kinase assays to assess β-catenin phosphorylation.

(A) β-Catenin-directed activity of an AXIN1-kinase complex containing either wild-type or polymerization-deficient (M3) AXIN1 ± APC, at different ATP concentrations. See Figure S7 for control reactions and representative autoradiographs. Data points are means from three independent experiments ± SEM.

(B) β-Catenin-directed kinase activity as in (A), but at different β-catenin concentrations.

(C) Summary of kinase activity parameters. Data are means from three independent experiments ± SEM. n.d., not determined because of lack of reaction velocity saturation.