FIG. 3.

PKR is implicated in G₀/G₁ arrest by ts p53. Shown is cell cycle analysis of PKR^+/+ and PKR^−/− clones expressing ts p53. Cells were incubated in DMEM–0.5% FCS for 48 h, refed with 10% FCS, and incubated at 32°C for the indicated periods of time. Cells were then fixed, stained with propidium iodide, and subjected to cell cycle analysis as described in Materials and Methods. M1, fraction of cells in G₀/G₁ phase; M2, cells in S phase; M3, cells in G₂+M.