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. 2021 Jul 20;14(7):1853–1866. doi: 10.14202/vetworld.2021.1853-1866

Table-7.

ASFV shedding in fecal samples posttreatment. Checking was performed up to 20 days of challenge. Individual pig samples were collected to screen for viral presence.

Day of challenge/Tag no Negative Control Treatment 1 NOBF (Direct challenge) Treatment 1 NOBF (Incubated challenge) Positive control




No challenge Challenge Cohoused Challenge Cohoused Challenge Cohoused







45 46 47 100 73 81 76 77 79 50 51 52 53 54 55 89 96 72 75 97
D-0 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
D-10 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
D-12 ND ND ND ND ND ND ND ND ND ND ND ND 35.89 36.92 ND ND ND
D-14 ND ND ND ND ND ND ND ND ND ND ND ND 30.72 32,26 ND ND ND
D-16 ND ND ND ND ND ND ND ND ND ND ND ND 29.29 31.52 36.21 ND ND
D-18 ND ND ND ND ND ND ND ND ND ND ND ND 27.31 30.26 35.93 36.1 ND
D-20 ND ND ND ND ND ND ND ND ND ND ND ND - 29.23 35.17 35.26 36.03

Ct value≥37 negative real-time PCR; ≤36=Positive real-time PCR; ND (not detected)=Negative real-time PCR. ASFV=African swine fever virus, NOBF=Natural oil blend formulation