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Spleen section showing: (a) Isotype control. (b) CD8 (CD4–CD8+) lymphocyte stained in brown color especially in red bulb (RP) 40×. (c) Lymphocytes expressing CD3 marker 100×. (d) Lymphocytes expressing Bu-1 marker 100×. Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.
