Table-3.

Determination of lymphocyte population subsets in lymphoid organs.

Cell phenotype	Thymus (%)	Spleen (%)	Bursa of Fabricius (%)
CD3+ (T-Cell)	24.4	62.3	2.04
Bu-1+ (B-Cell)	0.27	34.1	98.2
CD4+CD8- (T-helper)	0.36	16.2	0.39
CD4-CD8+ (Cytotoxic T-Cell)	3.91	39.5	0.2
CD4+CD8+	91	13.5	0.4
CD4-CD8-*	4.76	30.9	99
Total CD4+†	91.36	29.7	0.79
Total CD8+‡	94.91	53	0.6
Small mononuclear cells¦	73.1	60.2	58.4

Flow cytometry procedure has been applied for cell suspensions from thymus, bursa of Fabricius and spleen. Depending on the lymphocyte cell markers, the proportion of each cell was calculated in 8-week-old local breed male chickens (n=30).

^*Very immature thymocytes,

^†includes CD4⁺CD8^- and CD4⁺CD8⁺,

^‡includes CD4^-CD8⁺and CD4⁺CD8