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. 2021 Aug 30;7:225. doi: 10.1038/s41420-021-00619-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — For A to F: Thymic atrophy following the intrathymic injection of mDCs (n = 3). The same volume of PBS was used as a control (n = 3). A Thymus morphology and size. B Total numbers of thymocytes were calculated. C Thymocytes were stained with PE-conjugated anti-CD4 and APC-labeled anti-CD8 antibodies. The thymocyte subpopulations were examined by FCM. D The percentages and absolute numbers of indicated thymocyte subpopulations were calculated. E The cells isolated from the thymus of recipient mice were stained with Alexa Fluor 488-conjugated anti-CD45 and APC-labeled anti-EpCAM antibodies, respectively. CD45^-EpCAM⁺ TECs were detected by FCM. F The percentages and absolute numbers of CD45^-EpCAM⁺ TECs were calculated. All data are from three independent experiments. Representative figures of these three experiments are shown. Data are expressed as the mean ± SD. *P < 0.05, ***P < 0.001 compared with the control group.