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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Eur J Pharm Sci. 2021 Mar 27;162:105821. doi: 10.1016/j.ejps.2021.105821

Fig 5.

Fig 5.

Effect of harmine and harmol on glioma cancer cell cytotoxicity. For high content imaging, H4 and U87 cells were incubated with harmine (A) or harmol (B) at varying concentrations (0.1 – 66 μM) for 72 h and cells then stained with Hoechst and YOYO-1 dyes. Nuclear count, nuclear area and YOYO-1 staining were measured, data normalized to DMSO vehicle control and dose response curves generated by non-linear regression using GraphPad Prism 7. (C) Representative high content imaging fields (10x; overlay of Hoechst=blue, YOYO-1=green) for vehicle (DMSO), harmine (16 μM) and harmol (16 μM) on H4 and U87 cells. Dose response curves and images for the other harmine and harmol doses tested and for the other analogs are shown in the accompanying Data in Brief article (Tarpley et al. 2021b).