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. 2021 Aug 30;12:488. doi: 10.1186/s13287-021-02559-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — CD146 expression is associated with hMSC-DP proliferation, differentiation and immune modulation. a Western blot analysis showed the expression of CD146 in DPSCs and SHED at P5, P10 and P20 under SE or SF culture conditions. b The expression level of CD146 in DPSCs and SHED at P10 was downregulated by siCD146, as assessed by western blot. c Flow cytometry analysis showed the percentage of CD146-positive cells was reduced after siCD146 treatment. n = 3 for each group. d EdU staining showed the proliferation rate of hMSC-DP was decreased after siCD146 treatment. n = 3 for each group. e After co-culturing siCD146-transfected hMSC-DP with T cells, the percentage of apoptotic T cells was reduced, as assessed by flow cytometry. n = 3 for each group. f, g Osteogenesis of hMSC-DP was impaired by knocking down CD146, as assessed by the percentage of alizarin red staining (f) and western blot of osteogenic markers (g). n = 3 for each group. SE, serum; SF, serum-free. Data shown as mean ± SEM. **p < 0.01, ***p < 0.001. ns not significant