Fig. 1.
Experimental schematic representation of betacoronavirus MHV-3 as a model for SARS-CoV-2 to investigate the virucidal activity of equipment STR Solution®. Test model for the virucidal efficacy of STR Solution® simulating natural conditions at different heights, simulating the feet, abdomen, and head of a person 1.70 m (A). The virus stock was diluted and deposited onto the surfaces: polypropylene cut from Eppendorf tubes, stainless steel, fabrics and skin of Sus domesticus (B) at distances of 0.05 m and 3 m (C). The room temperature was controlled around 23 °C ± 2 and 50 ± 3 % relative humidity. The materials containing the viral particles were exposed at different time points (1 h, 3 h, and 5 h) to the STR solution® (D). The materials were eluted in microtubes containing FBS-free MEM and homogenized using a vortex type homogenizer for 5 s and subjected to sonication for 1 min (D). The viruses were tittered using series of the dilution (E). The 96 wells microplate was kept for 72 h, by incubation at 37 °C, 5% CO2, evaluating the cytopathic effect daily (F).