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esa1 mutants are temperature sensitive. Temperature sensitivity was observed by plating wild-type ESA1 (LPY3498) and esa1 mutant (esa1-L254P [LPY3500], esa1-L327S [LPY3430], and esa1-414 [LPY3291]) strains in fivefold serial dilutions at 28°C (left) and 37°C (right). All three alleles are recessive mutations.
