FIG. 6.

esa1 mutants display improper DNA segregation. ESA1 (LPY3498), esa1-L254P (LPY3500), and esa1-414 (LPY3291) cells were incubated at 37°C for 4 h, and esa1-L327S (LPY3430) cells were incubated at 37°C for 8 h, fixed, and stained with DAPI. esa1 mutant cells were predominantly large budded as visualized by Nomarski optics (left and center columns, bottom), with some triple-budded cells (right column, bottom). DAPI staining indicates that esa1 mutant cells either failed to segregate or abnormally segregated their chromatin in comparison to wild-type cells. Bar, 5 μm.