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. Author manuscript; available in PMC: 2021 Aug 30.
Published in final edited form as: Biochem Cell Biol. 2018 Nov 1;97(3):315–324. doi: 10.1139/bcb-2018-0142

Fig 4. Immunoprecipitation of CCVs using a Clathrin antibody attached to protein-G dynabeads.

Fig 4.

Protein-G Dynabeads, covalently attached to the X-22 Clathrin antibody, were incubated with CCVs and bound substrate was subsequently eluted. Shown are SDS-PAGE and Westerns with identical samples; the first Western was exposed to a Clathrin heavy chain antibody (α-Clathrin) and the second was exposed to the Synaptophysin antibody (α-syp). Lane 1 contains the unmodified CCV sample (starting CCVs), lane 2 is the CCV supernatant after Dynabead incubation (flow through), lane 3 contains a control of eluted antibody+Dynabeads (no CCV incubation) (control elution), and lane 4 contains the CCV sample eluted off the Dynabeads (CCV elution). Eluted antibody was visible in both Westerns and is marked.