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. 2021 Aug 26;9(8):e003132. doi: 10.1136/jitc-2021-003132

Figure 1.

Figure 1

Preparation and characterization of SVMAV. (A) Heatmaps showing the gene expression levels of JAK-STAT3 pathway-related genes and the top 40 upregulated genes by R848 in BMDCs based on transcriptome sequencing results. (B) Schematic of the OVA@SVMAV preparation. (C, D) Particle diameter distribution (C) and transmission electron microscope image of OVA@SVMAV (D). (E) Stability of nanoformulations with different compositions in PBS containing 10% FBS. (F, G) Release profiles of R848 and ova from R848-SS-DHA and OVA-CSSVVR-DHA, respectively, in the simulated intracellular environment. Data are displayed as mean±SD. BMDCs, bone marrow-derived dendritic cells; DTT, dithiothreitol; FBS, fetal bovine serum; OVA, ovalbumin; PBS, phosphate buffer saline; DHA, docosahexaenoic acid; JAK, janus kinase; CSSVVR, Cys·Ser·Ser·Val·Val·Arg; STAT3, signal transducer and activator of transcription 3; SVMAV, Self-assembling Vehicle-free Multi-component Antitumor nanoVaccine.