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. 2021 Aug 26;9(8):e003132. doi: 10.1136/jitc-2021-003132

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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SVMAV increased vaccine accumulation and DC uptake in lymph nodes in vivo. (A) Scheme of the experimental design. (B, C) Fluorescence imaging (B) and quantification of signals (C) of draining inguinal LNs at different time points after the subcutaneous injection of different formulations of vaccines. (D, E) Quantification data (D) and representative plots (E) for the flow cytometry analysis of FITC positive LN-residing DCs at 6 hours after different treatments. Data are displayed as mean±SD. **p<0.01,. DC, dendritic cell; dLNs, draining lymph nodes; FITC, fluorescein isothiocyanate; OVA, ovalbumin; SVMAV, Self-assembling Vehicle-free Multi-component Antitumor nanoVaccine.