Table 3. Normalized pH 6–induced inward peak currents carried by extracellular monovalent cations in hASIC1a WT and F4′A.

Test ion	Ix/INa (WT)	n	Ix/INa (F4′A)	n
K+	0.8 ± 0.5	11	0.6 ± 0.5	32
MA+	0.3 ± 0.1a	11	0.8 ± 0.5	46
DMA+	0.1 ± 0.1b	6	0.4 ± 0.3	32
EA+	0.2 ± 0.2	9	0.5 ± 0.4	47
DEA+	0.04 ± 0.02	5	0.02 ± 0.08	18
TEA+	−0.01 ± 0.0c	9	−0.03 ± 0.01	12
TetraEA+	−0.02 ± 0.03b	11	−0.04 ± 0.02	12
PA+	0.2 ± 0.1	5	0.5 ± 0.4	7
TetraPA+	−0.01 ± 0.4	11	−0.02 ± 0.02	9

Currents were recorded on a high-throughput patch-clamp robot (SynchroPatch 384PE). Currents carried by the different test ions were normalized to the mean Na⁺ current. Values are presented as mean ± SD. Currents carried by the test ions were compared with those carried by Na⁺ using an unpaired t test.

^aP < 0.001.

^bP < 0.05.

^cP < 0.0001.