Table 8. Single-channel conductances of WT hENaC and pre-M1 mutants.

Construct	Single channel conductance (pS)	n
WT hENaC	4.7 ± 1.0	6
α(F62A)βγ	4.5 ± 1.0	5
α(F62A)β(Y29A)γ	5.2 ± 0.3	5
α(T66V)βγ	7.1 ± 0.6a	7
α(T66V)β(T33V)γ	8.1 ± 0.6b	5

WT and mutant hENaC RNA was injected into Xenopus oocytes, and single-channel conductances were determined in the cell-attached patch-clamp configuration with 140 mM extracellular Na⁺. Single-channel conductances are presented as mean ± SD and were compared with WT ENaC using one-way ANOVA with Dunnett’s multiple comparisons test.

^aP < 0.05.

^bP < 0.05.