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. 2021 Aug 24;220(10):e202101019. doi: 10.1083/jcb.202101019

Figure 5.

Figure 5.

Remarkable translational alterations in TDPΔCR mice and HEK293ΔCR cells. (A) Representative EM micrographs and quantification showing changed ribosomal densities in hippocampal neurons of WT and TDPΔCR+/− mice (n = 18–20 neurons from 3 mice per group). (B) Schematic diagram of in vivo labeling of newly synthesized polypeptides by SUnSET in WT and TDPΔCR+/− mice. Lower panel: Representative images of newly synthesized polypeptide labeled by puromycin in the brains of WT mice. Lv, lateral ventricle; III, third ventricle; IV, fourth ventricle. (C) Representative immunoblot and quantification of newly synthesized polypeptide labeled by puromycin in the hippocampal tissues from WT and TDPΔCR+/− mice (n = 4 mice per group). (D) Representative EM micrographs and quantification showing changed ribosomal densities in WT and TDP-43ΔCR HEK293 cells (n = 11–12 cells per group). (E) Representative immunoblot and quantification of newly synthesized proteins labeled by Click-iT AHA in WT and TDP-43ΔCR HEK293 cells (n = 3 independent experiments per group). (F) Representative immunoblot and quantification of EGFP expression driven by cap-dependent (CMV-EGFP) and cap-independent (IRES-EGFP) translation in WT and TDP-43ΔCR cells (n = 3 independent experiments per group). Data are mean ± SEM; two-tailed Student’s t test. *, P < 0.05; **, P < 0.01; and ****, P < 0.0001.