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. 2021 Aug 25;218(10):e20210745. doi: 10.1084/jem.20210745

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© 2021 Gschwend et al.

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Figure 1. — Different hematopoietic cell populations contribute to GM-CSF production in the neonatal lung. (A) Gene-targeting strategy used to engineer Csf2^{flox-tdTomato} (Csf2^fl) mice. (B) Flow cytometry analysis of CD11c⁺SiglecF⁺ AMs in the lungs of adult Csf2^+/+, Csf2^+/fl, and Csf2^Δ/Δ mice, gated on CD45⁺ cells. (C) Flow cytometry analysis of tdTomato⁺ populations in the adult lungs of Csf2^+/+, Csf2^+/fl, and Csf2^+/Δ mice. (D) Flow cytometry of tdTomato expression in Csf2^+/+ and Csf2^+/fl P10 lungs, gated on CD45⁺ cells. (E) Percentage contribution from different hematopoietic cells types to the tdTomato⁺ (left) or tdTomato^bright compartment, as gated in D. (F) Expression of tdTomato in the indicated cell populations in lungs of P10 Csf2^+/+ (gray) and Csf2^+/fl (blue) mice was determined by flow cytometry analysis. Percentages (±SEM) of tdTomato⁺ cells are indicated. (G) MFI of tdTomato of Csf2^+/fl ILC2s, γδ T cells, and CD45⁻ cells in P10 lungs, gated on tdTomato⁺ cells as indicated in F. (H) Csf2 mRNA expression relative to Rps17 in the three major tdTomato⁺ cell populations isolated from P10 Csf2^+/fl lungs. (B–H) Data are from one experiment representative of two (B and C), five (D-G), or three (H) independent experiments. (G and H) ***, P 0.0001–0.001; ****, P < 0.0001. IRES, internal ribosome entry site; MFI, mean fluorescence intensity; rel., relative; UTR, untranslated region.