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. 2021 Aug 25;218(10):e20210745. doi: 10.1084/jem.20210745

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© 2021 Gschwend et al.

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Figure 3. — AT2s are the main nonhematopoietic source of GM-CSF in the neonatal lung. (A and B) Analysis of P10 lungs isolated from Csf2^+/+ (gray) and Csf2^+/fl (blue) mice. (A) Flow cytometry analysis of tdTomato expression in the CD45⁻ compartment. Isolated populations include AT2s (G1.1: CD45⁻CD31⁻EpCAM⁺MHCII⁺CD104⁻); airway epithelial cells (G1.2: CD45⁻CD31⁻EpCAM⁺MHCII⁻CD104⁺); fibroblasts (G2.1: CD45⁻CD31⁻EpCAM⁻PDGFRα⁺CD49f⁻); CD45⁻CD31⁻EpCAM⁻PDGFRα⁻CD49f⁻ cells (G2.2); CD45⁻CD31⁻EpCAM⁻PDGFRα ⁻CD49f⁺ cells (G2.3); and endothelial cells (G3: CD45⁻CD31⁺EpCAM⁻). (B) Flow cytometry analysis of tdTomato expression in the CD31⁻EpCAM⁺ compartment of fixed lungs, gated on CD45⁻ cells. Isolated populations include AT2s (G4.1: proSP-C⁺CD104⁻MHCII⁺; G6.1: NaPi-IIb⁺CD104⁻MHCII⁺), and airway epithelial cells (G5: proSP-C⁻CD104⁺; G7: NaPi-IIb⁻CD104⁺). (A and B) Data are from one experiment representative of two independent experiments.