Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 25;218(10):e20210745. doi: 10.1084/jem.20210745

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Gschwend et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — AT2-derived GM-CSF is necessary for the development of AMs in the neonatal lung. (A) Representative IF pictures of tdTomato⁺ cells (red) and CD45⁺ cells (green) in P10 lungs of Csf2^fl, SPC^Cre/+;Csf2^fl, and Csf2^Δ/Δ mice (scale bars, 20 µm). (B, C, E, and F) Analysis of P10 lungs isolated from Csf2^fl and SPC^Cre/+;Csf2^fl mice. (B) Flow cytometry analysis of the EpCAM⁺tdTomato⁺ population, gated on CD45⁻ cells. (C) Flow cytometry analysis of the Thy1.2⁺tdTomato⁺ population, gated on CD45⁺ cells. (D) Total GM-CSF quantification in lung conditioned media from P10 Csf2^fl, Vav1^iCre/+;Csf2^fl, SPC^Cre/+;Csf2^fl, and Csf2^Δ/Δ mice, ±SD. LD, limit of detection. (E) Flow cytometry analysis of CD11c⁺SiglecF⁺ AMs, gated on live CD45⁺Ly-6G⁻ cells. (F) Quantification of AMs. (A–E) Data are from one experiment representative of two (A and D) or four (B, C, and E) independent experiments. (F) Data are pooled from two independent experiments representative of four independent experiments. (D and F) *, P 0.01–0.05; **, P 0.01–0.001; ****, P < 0.0001.