Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 25;218(10):e20210745. doi: 10.1084/jem.20210745

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Gschwend et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S4. — Hematopoietic-derived GM-CSF is not critical for AM fate specification during embryogenesis. (A) Flow cytometry analysis of kinetics of tdTomato expression in perinatal lungs in Csf2^+/fl and Csf2^+/+ mice ranging from E14.5 to P4, gated on CD45⁺ cells. DOB, day of birth. (B–E) Analysis of E17.5 lungs isolated from Csf2^fl and SPC^Cre/+;Csf2^fl mice. (B) Flow cytometry analysis of tdTomato⁺ population, gated on CD45⁺ cells. (C) Gating strategy used to identify fetal macrophage and monocyte populations. (D) Flow cytometry analysis of Ly-6C and CD11c levels in the developing fetal monocyte population (G2 in Fig. 5 D). (E) Flow cytometry analysis of Ly-6C and CD64 levels in the developing fetal monocytes population (G2 in Fig. 5 D). (F–J) Analysis of E17.5 lungs isolated from Csf2^fl and Vav1^iCre/+;Csf2^fl mice. (F) Flow cytometry analysis of the tdTomato⁺ population, gated on CD45⁺ cells. (G) Flow cytometry analysis of the EpCAM⁺tdTomato⁺ population, gated on CD45⁻ cells. (H) Flow cytometry analysis of F4/80^high primitive macrophages (G1) and F4/80^int fetal monocytes (G2), gated on CD45⁺Ly-6G⁻CD64⁺MHCII⁻ cells. (I) Quantification of primitive macrophages (G1) and fetal monocytes (G2). (J) Flow cytometry analysis of Ly-6C and CD11b levels in the developing fetal monocytes population (G2). (K) Quantification of Ly-6C^high (G2.1), Ly-6C^int (G2.2), and Ly-6C^low (G2.3) fetal monocytes. (A) Data are representative of at least two independent experiments per time point. (B–H and J) Data are from one experiment representative of three (B–E) or two (F–H and J) independent experiments. (I and K) Data are pooled from two independent experiments. ns, P ≥ 0.05; **, P 0.01–0.001.