Figure 4.

Cholesterol esterification is necessary for remyelination. (A) Representative images of azure blue staining of semithin sections from 21-dpi lesions from WT and ACAT KO mice. (B and C) The quantification of foam cells (B) and remyelinated axons (C). (D–F) Confocal images (D) and quantification (E) of 21-dpi lesions showing increased density of inflammatory cells (IBA1⁺, magenta) in ACAT KO mice compared with the WT control. The number of phagocytes with undigested myelin (arrowheads in D labeled with IBA1, magenta; FM, green; and LAMP1, red) was similar between WT and ACAT KO animals; quantification shown in F. Scale bars, 15 μm in A and 50 μm in D. n = 3–4 mice per condition for all the experiments. Data represent mean ± SEM. P values were calculated using two-tailed unpaired t test. *, P < 0.05; **, P < 0.01.