Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Ctk2p turnover in late exponential cells. Δctk2 cells transformed with the CTK2-HA construct were grown in SD medium to an OD600 of 3. Aliquots were taken at different times (minutes) of incubation at 30°C after the addition of cycloheximide. Resulting cellular extracts were analyzed by Western immunoblotting. T+, protein extracts from Δctk2 cells transformed with the CTK2-HA construct grown to an OD600 of 0.8.
