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Ctk2p phosphorylation in Δctk1 cells. Δctk1 cells transformed with the CTK2-HA construct were grown in SD medium to an OD600 of 0.8. Aliquots were taken at different times (minutes) of incubation at 30°C after the addition of cycloheximide. Resulting cellular extracts were analyzed by Western immunoblotting. As a control (T+), protein extracts from Δctk2 cells transformed with the CTK2-HA construct grown to an OD600 of 0.8 were loaded on the gel.
