FIG 4.

MS-rings purified from cells coexpressing FliF with FlhF and/or FliG. (A to C) EM image of MS-rings isolated and purified from E. coli BL21(DE3) cells coexpressing FliF and FlhF in pRO101 and pTSK122 plasmids (A), coexpressing FliF with FliG in pTSK137 plasmid (B), and coexpressing FliF with FliG and FlhF in pTSK137 and pTSK122 plasmids, respectively (C). Bars, 100 nm. The embedded upper left panel in each image is an enlarged image of the MS-ring (bars, 50 nm). The grid was stained using uranyl acetate.