TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or features | Reference or source |
|---|---|---|
| P. aeruginosa strains | ||
| PAKa | Wild type | 31 |
| AJDP730 | ΔctpA | 17 |
| AJDP1228 | ΔmepM | 14 |
| AJDP1229 | ΔctpA ΔmepM | 14 |
| AJDP1264 | Δprc | This study |
| AJDP1265 | ΔctpA Δprc | This study |
| AJDP1385 | Δprc Δ(PA1198-PA1199) | This study |
| AJDP1386 | ΔctpA Δprc Δ(PA1198-PA1199) | This study |
| AJDP1390 | ΔctpA Δ(PA1198-PA1199) | This study |
| Plasmids | ||
| pHERD20T | Ampr, pMB1 ori, araBp expression vector | 32 |
| pET-24b(+) | Kanr, pMB1 ori, T7p expression vector | Novagen |
| pEX18Ap | Ampr, pMB1 ori, oriT, sacB+ | 33 |
| pQE-30 | Ampr, Col E1 ori, T5p expression vector | Qiagen |
| pAJD2290 | T7p-´ctpA-his6 in pET-24b(+) | 17 |
| pAJD2653 | T7p-´lbcA-his6 in pET-24b(+) | 14 |
| pAJD2655 | T7p-´ctpA-S302A-his6 in pET-24b(+) | 14 |
| pAJD2799 | araBp-mepM-FLAG in pHERD20T | 14 |
| pAJD2805 | araBp-mepM in pHERD20T | This study |
| pAJD2897 | araBp-PA1199 in pHERD20T | This study |
| pAJD2909 | araBp-mepM-Δ6 in pHERD20T | This study |
| pAJD2912 | araBp-PA1199-Δ7 in pHERD20T | This study |
| pAJD2929 | araBp-mepM-Δ9 in pHERD20T | This study |
| pAJD2930 | araBp-mepM-Δ8 in pHERD20T | This study |
| pAJD2931 | araBp-mepM-Δ7 in pHERD20T | This study |
| pAJD2935 | araBp-PA3787 in pHERD20T | This study |
| pAJD2936 | araBp-PA3787-MepMCT11 in pHERD20Tb | This study |
| pAJD2939 | araBp-PA1199-Δ6 in pHERD20T | This study |
| pAJD2940 | araBp-PA1199-Δ5 in pHERD20T | This study |
| pAJD2941 | araBp-PA1199-Δ4 in pHERD20T | This study |
| pAJD2946 | T5p-his6-′mepM in pQE-30 | This study |
| pAJD2947 | T5p-his6-′mepM-Δ7 in pQE-30 | This study |
| pAJD2951 | T5p-his6-′PA1199 in pQE-30 | This study |
| pAJD2952 | T5p-his6-′PA1199-Δ7 in pQE-30 | This study |
| pAJD2953 | araBp-PA1199-Δ7-MepMCT7 in pHERD20Tc | This study |
| pAJD2955 | araBp-mepM-Δ7-PA1199CT7 in pHERD20Td | This study |
| pAJD2971 | araBp-PA1199-Δ7-AGEAGHL in pHERD20T | This study |
| pAJD2972 | araBp-mepM-Δ7-AGEAGHL in pHERD20T | This study |
| pAJD2982 | araBp-mepM lbcA-FLAG in pHERD20T | This study |
| pAJD2983 | araBp-mepM-Δ7 lbcA-FLAG in pHERD20T | This study |
| pAJD2984 | araBp-mepMEC-FLAG in pHERD20Te | This study |
| pAJD2985 | araBp-mepMEC-MepMCT11-FLAG in pHERD20Tf | This study |
| pAJD2992 | araBp-PA1199 lbcA-FLAG in pHERD20T | This study |
| pAJD2993 | araBp-PA1199-Δ7 lbcA-FLAG in pHERD20T | This study |
a
All P. aeruginosa strains are derivatives of strain PAK.
b
PA3787 with the C-terminal 11 amino acids of MepM added to its C terminus.
c
PA1199 with its C-terminal 7 amino acids replaced by those of MepM.
d
MepM with its C-terminal 7 amino acids replaced by those of PA1199.
e
mepMEC is the E. coli mepM gene.
f
E. coli MepM with the 11 C-terminal amino acids of P. aeruginosa MepM added to its C terminus.