TABLE 6.

Transformation assay of TLS^a

Recipient strain	Genotype	Control DNAb		oxoG DNA		Transformation efficiency		No. inserted at oxoGe
		No. of EPc	No. of transformants	No. of EP	No. of transformants	Relative to controld	Relative to WT	C	A
MR31	ogg + dbh +	7	162	10	44	0.181	1	27	0
SD67-7	Δogg	7	72	37	61	0.160	0.89	31	0
RJ4	Δdbh	30	219	185	23	0.017	0.094	1	18
RJ8	Δogg Δdbh	11	63	127	30	0.041	0.23	1	27

^aBased on OMT, as described in Materials and Methods.

^bControl indicates an intact (normal) oligonucleotide, used for normalization.

^cNumber of electroporations (EP) performed (770 pmol DNA each).

^dRatio of transformants per pmol oxoG-containing DNA to transformants per pmol control DNA.

^eThe numbers represent transformants of each type generated by oxoG-containing DNA.