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. 2020 Jul 27;202(16):e00202-20. doi: 10.1128/JB.00202-20

FIG 1.

FIG 1

The M. tuberculosis Δrv3679-80 mutant is hypersusceptible to NO and exhibits a glycerol-dependent recovery defect following mouse infection. (A) Survival in the presence of 2 mM sodium arsenite. (B) Survival of bacteria treated with diethylenetriamine NONO-ate (DETA-NO) in standard 7H9 liquid medium and cultured on standard 7H10 agar for CFU enumeration. (C) Images of bacterial colonies cultured on standard 7H10 agar (0.5% glycerol, 10% OADC) from diluted infection inoculum and from lung homogenates at day 63 postinfection. Images were taken at the indicated incubation time points. (D) Images of bacterial colonies cultured on glycerol-free 7H10 agar (0.5% glucose, 10% OADC). (E) Survival of bacteria treated with DETA-NO in glycerol-free 7H9 liquid medium and cultured on glycerol-free 7H10 agar for CFU enumeration. Data in panels B and E are means and errors from three independent experiments; error bars indicate standard deviation. Statistical significance of the Δrv3679-80 mutant relative to the WT was assessed by Student's t test; *, P < 0.05; **, P < 0.01.