FIG 3.

Intact ATP-binding motifs are required for WT levels of resistance to elevated glycerol and NO. (A) Growth of indicated strains on agar plates containing 0.5% glycerol (left) or 5% glycerol (right). (B) Growth in liquid culture in the presence of 0.2% glycerol (left) or 5% glycerol (right). Data are representative of 3 independent experiments. (C) Survival following treatment of strains with 20 μM DETA-NO in medium with 0.2% glycerol. Data are means from 3 independent experiments; error bars indicate standard deviation (SD). One-way ANOVA with Dunnett’s multiple comparison correction at each time point to compare all strains to WT (**, P < 0.01).