FIG 5.

The M. tuberculosis Δrv3679-80 mutant is hypersusceptible to extracellular methylglyoxal but does not accumulate it more than the WT. (A) Intrabacterial pool sizes of methylglyoxal derivative product 2-methylquinoxaline (2-MQ) in the indicated strains after 3 days of cultivation on 7H10 agar with 0.2% glucose and either 0.5% or 5% glycerol. Pool sizes are expressed as area under the curve normalized to protein content. Data from triplicate cultures are shown relative to the WT input values. *, P < 0.05; **, P < 0.01 by one-way ANOVA with Bonferroni multiple comparison. (B) Impact of methylglyoxal on growth of the indicated strains. Data are means from two independent experiments each with triplicate cultures; error bars indicate SEM.