TABLE 1.
Identity of glycerol-resistant transposon mutants
| Mutant code(s)d | Gene | Protein (length)a | No. of times isolated | Consequence of transposon insertion | glpK mutationc | Spontaneous GlpK mutation |
|---|---|---|---|---|---|---|
| A1, A4, A6, B3, B4, C6, D3 | rv3696c | GlpK (517 aa) | 7 | Premature stop (aa 316) | Y | N/A |
| C3, D2 | rv3696c | GlpK (517 aa) | 2 | Premature stop (aa 230) | Y | N/A |
| D4 | rv3696c | GlpK (517 aa) | 1 | Premature stop (aa 350) | Y | N/A |
| B6, C2 | Intergenic nt 4139762 | N/Ab,e | 2 | N/A | Y | N/A |
| A2 | rv2954c | Hypothetical protein (241 aa) | 1 | Premature stop (aa 142) | Y | Homopolymeric region expansion (G191) resulting in premature stop (aa 252) |
| A5 | rv0831c | Hypothetical protein (271 aa) | 1 | Frameshift (aa 211) | Y | G36C |
| B1, B2, B5 | rv2178c | AroG (462 aa) | 3 | Addition of 14 aa | Y | G36C |
| D1 | rv3273 | Probable carbonic anhydrase (764 aa) | 1 | Frameshift (aa 421) | Y | Homopolymeric region expansion (G191) resulting in premature stop (aa 252) |
| D5 | rv3049c | Probable monooxygenase (524 aa) | 1 | Premature stop (aa 492) | Y | Homopolymeric region expansion (G191) resulting in premature stop (aa 252) |
| C5, D6 | rv2370c | Hypothetical protein (437 aa) | 2 | Frameshift (aa 364) | N | N/A |
a
aa, amino acid(s); TSS, transcription start site.
b
N/A, not applicable.
c
Y, yes; N, no.
d
Mutant codes refer to the agar plates (A to D) and colonies (1 to 6) picked and analyzed.
e
Mutation is 6 bp upstream of the TSS of glpK (39).