TABLE 2.
Strains used in this work
| Species | Strain | Source or reference | Additional information |
|---|---|---|---|
| M. tuberculosis | Wild-type H37Rv | Gift from C. Sassetti, University of Massachusetts | |
| M. tuberculosis | Δrv3679-80 mutant | This work | WT background, hygromycin resistance cassette replaces rv3679 and rv3680, kanamycin resistance (Kanr) cassette at attL5 site |
| M. tuberculosis | Δrv3679-80::rv3679-80 mutant | This work | Δrv3679-80 mutant background, Phsp60-rv3679-rv3680 with kanamycin resistance at attL5 site |
| M. smegmatis | mc2155 | ATCC | |
| M. smegmatis | Δmsmeg_6193-95 mutant | This work | mc2155 background, hygromycin resistance cassette replaces msmeg_6193 and msmeg_6195 |
| M. smegmatis | Δmsmeg_6193-95::msmeg_6193_95 mutant | This work | Δmsmeg_6193-95 mutant background, Phsp60-msmeg_6193-95 |
| M. smegmatis | Δmsmeg_6193-95::rv3679-80 mutant | This work | Δmsmeg_6193-95 mutant background, Phsp60-rv3679-FLAG, phsp60-rv3680-HA |
| M. smegmatis | ΔclpB mutant | 15 | MsmΔmsmeg_0732::hyg, courtesy of Julien Vaubourgeix and Carl Nathan |
| M. tuberculosis | Δrv3679-80:: rv3679-FLAG, rv3680-HA mutant | This work | Δrv3679-80 mutant background, phsp60-rv3679-FLAG at attL5 site, phsp60-rv3680-HA at Giles site |
| M. tuberculosis | Δrv3679-80:: rv3679-K34A-FLAG, rv3680-HA mutant | This work | Δrv3679-80 background, Phsp60-rv3679-K34A-FLAG at attL5 site, Phsp60-rv3680-HA at Giles site |
| M. tuberculosis | Δrv3679-80:: rv3679-FLAG, rv3680-K32A-HA mutant | This work | Δrv3679-80 background, Phsp60-rv3679-FLAG at attL5 site, Phsp60-rv3680-K32A-HA at Giles site |