Figure 3. Death of H460 cells exposed to adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), ASC-interferon (IFN)-β, or their conditioned medium (CM). A: Death of H460 cells indirectly co-cultured with ASCs, ASC-TRAIL, or ASC-IFN-β for 2 days was evaluated via phycoerythrin (PE)-annexin-V and 7-amino-actinomycin D (7-AAD) staining. Annexin-V-positive/7-AAD-negative, annexin-V-positive/7-AAD-positive, and annexin-V-negative/7-AAD-positive cells were considered dead cells. Data are expressed as the mean±SD from three independent experiments. B: Caspase-3 activation in H460 cells treated with the CM from ASCs, ASC-TRAIL, and ASC-IFN-β for 2 days. The expression of cleaved caspase-3 was detected by immunoblotting. The data are expressed as the mean±SD from three independent experiments. Significantly different at: *p≤0.05.