Figure 3. The effect of cisplatin and pemetrexed on DNA fragmentation. A: A549 cells were stained with fluorescein isothiocyanate-dUTP dye after incubation with cisplatin (200 nM) and pemetrexed (100 nM) as single-agent therapy and combined therapy for 72 h. The fluorescence signal was measured by flow cytometry. (B) The bar diagram shows the quantification of DNA fragmentation. The results were expressed as the mean±SD from three independent experiments. ***Significantly different at p<0.001 vs. control by one-way analysis of variance followed by Dunnett’s multiple comparison test.
