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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Connect Tissue Res. 2020 Oct 12;62(6):615–628. doi: 10.1080/03008207.2020.1828380

Figure 2:

Figure 2:

RTL3 is expressed in human tissues and cell lines and the expression is reduced in osteoarthritic cartilage and upon RTL3-depletion. (A) qPCR analysis showed that RTL3 mRNA was expressed in several human tissues and cell lines. Expression of RTL3 mRNA (B) and protein levels (C) were significantly lower in osteoarthritic cartilage compared to normal (no history of rheumatic disease) control. (D) GeneQuery qPCR assays detected significantly increased mRNA expression of MMP-13, ADAMTS4 and ADAMTS5 in OA cartilage compared to normal. Treatment with IL-1β significantly reduced RTL3 mRNA expression in both normal and OA chondrocytes (E). RTL3 depletion was successful in chondrocytes and resulted in significantly reduced mRNA (F) and protein levels (G) without adversely affecting cell viability (H).