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Over expression of RTL3 in HEK-293T cells, that do not express RTL3, increased ectopic SOX-9 and COL2A1 mRNA expression and increased activity of the COL2A1 promoter reporter. RTL3 was successfully over expressed in HEK-23T cells (A) without affecting cell viability (B). RTL3 over expression significantly increased activity of the COL2A1 promoter reporter (C) and mRNA expression of SOX-9 (D) and COL2A1 (E).
