Figure 10.
Pharmacological inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly- adenosine diphosphate-ribose polymerase (TIPARP) increases aryl hydrocarbon receptor (AHR) signaling in mouse embryonic fibroblasts (MEFs) isolated from Tiparp+/+ but not TiparpH532A mice. Increased TCDD-induced mRNA expression levels of (A) Cyp1a1, and (B) Cyp1b1 in MEFs isolated from TiparpH532A and Tiparp+/+ mice treated with TCDD alone or in combination with increasing concentrations of RBN-2397. Real time-quantitative PCR data are shown relative to the gene-specific expression level observed for TiparpH532A MEFs exposed to 1 nM TCDD. *p < .05 by 1-way analysis of variance followed by Tukey’s post hoc test compared with genotype-matched TCDD-treated MEFs. Figure depicts the mean ± SEM (n = 3).