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. 2021 Jun 15;183(1):154–169. doi: 10.1093/toxsci/kfab075

Figure 5.

Figure 5.

Increased hepatic gene expression of aryl hydrocarbon receptor (AHR)-regulated genes. Mice were treated with a single injection of 10 µg/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or corn oil (CO) (vehicle) alone, and euthanized 6 days later. RNA and real time-quantitative PCR for (A) Cyp1a1, (B) Cyp1b1, (C) Nqo1, (D) Tiparp, (E) Ahrr, and (F) Fgf21 were performed as described in the materials and methods section (n = 5). G, Representative western blot (n = 3) of hepatic AHR protein levels in Tiparp+/+ and TiparpH532A mice 6 days after treatment with 10 µg/kg TCDD or CO (vehicle). *p < .05 2-way ANOVA followed by Sidak’s post hoc test compared with genotyped-matched control-treated mice. #p < .05 2-way ANOVA followed by Sidak’s post hoc test compared with time-matched TCDD-treated Tiparp+/+ mice. Figure depicts the mean ± SEM.