. 2021 Aug 10;15(8):e0009657. doi: 10.1371/journal.pntd.0009657

Table 2. QUADAS-2 Assessment of bias.

Studies designed with objective to assess the validity of the 20WBCT
First author, year of Publication	Was a consecutive or random sample of patients enrolled?	Was a case control design avoided?	Did the study use appropriate exclusion criteria?	Was the 20WBCT interpreted without knowledge of reference standard?	Was the 20WBCT conducted using standardized technique?	Was the reference standard, its conduct, or its interpretation performed in a manner to avoid bias?	Did all patients receive the same reference standard?	Was the reference standard performed on site and not require freezing?	Were all patients accounted for in the analysis?
Thongtonyong, 2020	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Tongpoo, 2020	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
DSilva, 2019	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Saengnoi, 2019	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Unknown$	$No$
Silva, 2018	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$No$	$Yes$
Ratnayake, 2017	$Yes$	$Yes$	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Paiva, 2015	$Unknown$	$Unknown$	$Unknown$	$Yes$	$Yes$	$Unknown$	$Yes$	$Yes$	$Unknown$
Biradar, 2015	$Yes$	$Yes$	$Yes$	$Unknown$	$Unknown$	$Unknown$	$Yes$	$Unknown$	$Yes$
Pongpit, 2012	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$No$	$Yes$
Sano-martins, 1994	$Unknown$	$Yes$	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Studies that were not specifically designed with the objective of assessing the validity of 20WBCT
Bhatt, 2020	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Wijewickrama, 2020	$Unknown$	$Yes$	$No$	$Yes$	$Unknown$	$Yes$	$Yes$	$Yes$	$Yes$
Sharma, 2020	$Yes$	$Yes$	$Yes$	$No$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Shenoy, 2020	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$
Oliveira, 2019	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$Yes$	$No$	$Yes$